What to do if the blank test is high when using the Kjeldahl method to measure protein content?
The Kjeldahl method is a commonly used technique for determining the total nitrogen content in compounds or mixtures. Due to its accuracy, it is still regarded as a standard testing method today. The
The Kjeldahl method is a commonly used technique for determining the total nitrogen content in compounds or mixtures. Due to its accuracy, it is still regarded as a standard testing method today. The Kjeldahl nitrogen analyzer, which is popular on the market, is highly praised, but various issues may arise during the experiment, leading to a series of detection anomalies. When using the Kjeldahl method to measure protein content, blank tests may show higher values. What are the potential causes for this?
First, it’s essential to check the purity of the reagents used, including distilled water, boric acid, and sodium hydroxide, to ensure they are of analytical grade. These basic testing conditions must be met. If there is any issue with the reagents, replace them promptly and retest.
Second, blank tests may introduce nitrogen-containing impurities. This emphasizes the need for carefulness during the experiment, ensuring that all apparatus is in a clean, dry state and that reagents are not cross-contaminated.
Third, the containers involved in the experiment may have been contaminated and not cleaned properly. For example, the anti-splash tube on the digestion tube may accumulate dirt after prolonged distillation. To clean it, a common method is to pour half a portion of boric acid into the digestion tube, heat the solution to dissolve any residues, and then distill for about 10 minutes. Afterward, distill several times with distilled water to further clean the system.
These are the basic analyses of the reasons why blank test values might be high during Kjeldahl nitrogen testing. Of course, if problems arise during the experiment, the causes should be identified step by step. In conclusion, reliable conditions and careful procedures are key to achieving satisfactory results in the experiment.
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